IN VITRO PLANT REGENERATION IN LOCAL AUS RICE THROUGH MATURED SEED CULTURE

An investigation was carried out to decipher the effect of genotypes and concentrations growth regulators for callus induction subsequent plant regeneration from mature seeds rice. Two local rice cultivars viz. Nayanmony Kalomona two 2, 4-D NAA were used evaluate their performance. The cultured under aseptic condition onto MS basal medium at varying combinations (1.0, 1.5 2.0 mgL- 1) (0.0, 0.5 1.0 mgL-1) induction. For fortified with 1 3.0 mgL-1 BAP applied. Between showed better responses in frequency (91.38%), weight (0.90 g)as well as diameter (7.48 mm). Among (1.5 alone produced best (95.16%) highest (1.88 g) but (8.27 mm) +0.5 NAA. It observed that both genotype regulator significantly affected regeneration. supplemented + NAA0.0 exhibited performance (99.33%) (1.92 cultivar whereas (8.38 NAA0.5 mgL-1. performed regarding (88.15%) compared (86.67%). Plantlets derived mgL-1+ 0.0 results survival. Therefore, it is suggested application 2,4-D advantageous accomplish overall efficiency induction, survival cultivars. Thus, selection responsive like would be beneficial transformation attempts improve crop.